Hệ thống quản lý khoa học - Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at AWildlife

Tên bài báo:	Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at AWildlife–Livestock Interface in Lusaka, Zambia (Lượt xem: )
Tên tạp chí:	Antibiotics
Tạp chí thuộc:	Tạp chí KH quốc tế ISI
Chỉ số IF/H:	3.893
Năm xuất bản:	2021
Nước xuất bản:	Thụy Sỹ
Tác giả thuộc Học viện:	PGS.TS. Mai Thị Ngân
Các tác giả bài báo:	Emmanuel Kabali, Girja Shanker Pandey, Musso Munyeme, Penjaninge Kapila, AndrewNalishuwa Mukubesa, Joseph Ndebe, John Bwalya Muma, Charles Mubita, Walter Muleya,Elizabeth Muligisa Muonga, Shuya Mitoma, Bernard Mudenda Hang’ombe, Anuwat Wiratsudakul, Mai Thi Ngan, Eslam Elhanafy, Hala El Daous, Nguyen Thi Huyen, Wataru Yamazaki,Tamaki Okabayashi, Maiku Abe, Junzo Norimine, Satoshi Sekiguchi
Tóm tắt bài báo:	A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife–livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern.
Link:	10.3390/antibiotics10030238